SHMT2 Knockout cell line (MDA-MB-231)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

SHMT2 Knockout cell line (MDA-MB-231) Order

Catalog#

YKO-H866

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

SHMT2 Knockout cell line (MDA-MB-231)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

SHMT2

Gene ID

6472

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)

Target region

Knockout region: Exon 4 (based on transcript SHMT2-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:MDA-MB-231			STR Info (Cell bank) Cell Line:MDA-MB-231
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	13	13		13	13
D7S820	8	9		8	9
D16S539	12	12		12	12
VWA	15	18		15	18
TH01	7	9.3		7	9.3
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	12	13		12	13

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SHMT2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MDA-MB-231 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SHMT2 gene knockout SHMT2 Knockout cell line (MDA-MB-231) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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SHMT2 Knockout cell line (MDA-MB-231)

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SHMT2 Knockout cell line (MDA-MB-231) Order

Detailed Product Information

Instruction

Related Popular Products

Wild-type Cell Lines

Human Breast Cancer Cell Line(MDA-MB-231) More details Order

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit More details Order

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium More details Order

KO Cell Lines

Shmt2 Knockout cell line(C2C12) inquiry More details

SHMT2 Knockout cell line (Hela) inquiry More details

SHMT2 Knockout cell line (HCT 116) inquiry More details

Related Popular Products

Wild-type Cell Lines

Human Breast Cancer Cell Line(MDA-MB-231)

Mouse Genotyping Kit

High-performance Mouse Genotyping Kit

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

KO Cell Lines

Shmt2 Knockout cell line(C2C12)

SHMT2 Knockout cell line (Hela)

SHMT2 Knockout cell line (HCT 116)

Gene Editing Services

EZ-editor™ Products

Contact us✖

Human Breast Cancer Cell Line(MDA-MB-231)
More details Order

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More details Order

EZ-cryo™ Cell Freezing Medium
More details Order

Shmt2 Knockout cell line(C2C12)
inquiry More details

SHMT2 Knockout cell line (Hela)
inquiry More details

SHMT2 Knockout cell line (HCT 116)
inquiry More details